Our results suggested that the transformation protocol will provide an effective tool for breeding of seashore paspalum in the future.Īrun M, Subramanyam K, Mariashibu TS, Theboral J, Shivanandhan G, Manickavasagam M, Ganapathi A (2015) Application of sonication in combination with vacuum infiltration enhances the Agrobacterium-mediated genetic transformation in Indian soybean cultivars. GUS staining experiments showed that the GUS gene was expressed in transgenic plants. PCR and Southern blot analysis showed that T-DNA was integrated into the genomes of seashore paspalum. By using the optimized transformation conditions, transgenic seashore paspalum plants were obtained. The results showed that a high transient transformation efficiency was observed by using Agrobacterium concentration of OD 600 = 0.6, 5 min of sonication treatment during Agrobacterium infection, and 2 d of co-cultivation. Three clones of callus were selected for examining transformation conditions using Agrobacterium tumefaciens strain AGL1 carrying the binary vector pCAMBIA1305.2, containing β-glucuronidase ( GUS) as a reporter gene and hygromycin phosphotransferase ( HPT) as a selective marker gene. Based on establishment of embryogenic callus induction and regeneration from different mature seeds of ‘Sea Spray’, an Agrobacterium tumefaciens-mediated transformation was established and optimized in this study.
Swartz) is an important warm-season turfgrass with great salinity tolerance. Seashore paspalum ( Paspalum vaginatum O.
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